WebHence to understand functions of tetraspanin proteins, it is critical to identify laterally interacting partner proteins. Here we used a novel technical approach involving exposure and cross-linking of membrane-proximal … WebFeb 23, 2024 · The cysteine pairs reported on the conformation of the TM helices that line the lateral openings, TM2-TM11 and TM5-TM8 (Fig. 1c, d ). We screened for successful cross-links by using a gel-shift...

Chemo-Selective Cys-Pen Disulfide for Proximity-Induced …

WebMar 31, 2009 · Cysteine–cysteine cross-linking at a C-terminal position such as Y113C, which is a predicted interfacial position, would bring the C-terminal parts of the dimer into … WebAug 28, 2001 · By using cysteine cross-linking, we have now explored the possibility that DAT exists as a dimer or higher-order oligomer in the plasma membrane. We show that DAT is at least a homodimer, with the extracellular end of TM6 at a symmetrical dimer interface. bobby orr and donald trump

A catalytically essential motif in the external loop 5 of the bacterial ...

WebIn contrast to copper-containing enzymes in which copper is required for activity, for cysteine dioxygenase it has been shown that the noncross-linked enzyme has low initial activity and that enzyme activity increases after crosslink formation. 66 It has been proposed that the cysteine-tyrosine cross-link formation properly positions the ... WebWe used systematic cysteine cross-linking in the TMH region of Ire1 to gain insight into the structural organization of signaling-active clusters during ER stress. WebMar 31, 2009 · Cysteine–cysteine cross-linking at a C-terminal position such as Y113C, which is a predicted interfacial position, would bring the C-terminal parts of the dimer into contact, forcing the dimer to take the closed form even in the absence of cholesterol. bobby orr all star card