2024 Fastgene gel/pcr extraction kit 300preps

Fastgene gel/pcr extraction kit 300preps

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August undefined, 2024

WebProduct Details. The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA … WebJul 20, 2024 · Use QIAquick gel extraction kit by Qiagen, it works well. Just use half of the elution buffer in coulmns in the final step of eluting the DNA and wait 3 minutes after …

FastGene Gel/PCR Extraction Kit - NIPPON Genetics EUROPE

WebThe PureLink Quick Gel Extraction and PCR Purification Combo Kit is designed to purify DNA fragments from agarose gels. The simple procedure uses a silica-based spin cartridge to purify DNA fragments from 40 bp to 10kb in <30 minutes from TAE or TBE agarose of various percentages and melting points. The PCR purification protocol achieves rapid ... WebCleanNGS SPRI Bead Purification Kit (277 clean-ups) 5 ml CNGS005 $124.69 PCR & Gel Extraction Kits FastGene Gel/PCR Extraction Kit 100 preps FG-91202 $194.51 Plasmid Purification Kit Fast DNA-spin Plasmid Purification Kit 200 preps 17013 $236.25 DNA-spin Plasmid Purification Kit 200 preps 17098 $162.75 DNA-maxi Endotoxin-Free … girft orthopaedics report

QIAquick Gel Extraction Kit and QIAquick PCR & Gel Cleanup Kit

WebFastGene ® plasmid DNA purification kits for the purification of high-copy and low-copy plasmids from bacterial over-night cultures. ... Gel/PCR Purification. Magnetic Stands. For 1.5 ml Tubes. For 96-well Plates. For … Web740609.250. NucleoSpin® Gel and PCR Clean-Up. 250 Preps. USD $447.00. NucleoSpin Gel and PCR Clean-up (250) 250 preps for PCR-clean-up and gel extraction - NucleoSpin and PCR Clean-up Columns, … Web1.3 Cloning of the kelch13 PCR fragment ... purified using the FastGene Gel/PCR Extraction Kit (Nippon Genetics, Co., Ltd., Tokyo, Japan), and fused with the In-Fusion HD Cloning Kits (Takara fun and silly

Nucleic Acid Purification - NIPPON Genetics EUROPE

FG-91302- FastGene Gel/PCR Extraction Kit (300 preps)

WebJun 22, 2024 · PCR products from the previous reactions were purified from an agarose gel using a FastGene Gel/PCR Extraction Kit (Nippon Genetics, Tokyo, Japan) and used as templates for mass amplification using TB71/CI78. sgRNA was synthesized and purified using the same kit. The concentration of the DNA template and the sgRNA were … WebPCR & Gel Extraction FastGene Gel/PCR Extraction Kit 100 preps FG-91202 $195 MEGAquick-spin plus Fragment DNA Purification Kit (from 87bp – 20kb) 200 preps … girft northern irelandWebMay 30, 2024 · PCR products were purified using a FastGene Gel/PCR Extraction Kit (Nippon Genetics). These samples were subjected to cycle sequencing using a BigDye™Terminator Cycle Sequencing ... A paired-end library was constructed with a TruSeq DNA PCR-Free Library Prep kit (Illumina, CA, USA) and was size-selected on … fun and shakes halifax

"WebFABGK 001-2. Blood Genomic DNA Extraction Mini Kit , (With Proteinase K Powder, for wide applications and simple procedure) 300preps. 416.23 €. -. Favorgen. FABGK 003. Blood Genomic DNA Extraction MAXI Kit (With Proteinase K Powder, for wide applications and simple procedure) 10preps. " - Fastgene gel/pcr extraction kit 300preps

Fastgene gel/pcr extraction kit 300preps

Is it necessary to gel purify your PCR product prior to cloning?

WebThe purification and preparation of nucleic acids forms an important basis for many molecular biological analysis, in research institutes as well as medical laboratories. Our kits deliver high-quality nucleic acids (RNA, viral RNA/DNA, plasmid DNA, gel and PCR fragments) quickly and reliably. They can directly be used in subsequent applications. WebQIAquick PCR Purification Kit QIAquick Nucleotide Removal Kit QIAquick Gel Extraction Kit. QIAGEN Distributors ... QIAquick Gel Extraction Kits (50) (250) Catalog No. 28704 28706 QIAquick Spin Columns 50 250 Buffer QG* 2 x 50 ml 2 x 250 ml Buffer PE (concentrate) 2 x 10 ml 2 x 50 ml

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WebApr 27, 2024 · The FastGene ® RNA kits were developed for the rapid, efficient and clean purification of RNA from tissues and cells. The silica … WebQIAquick PCR Purification Kit, QIAquick Nucleotide Removal Kit, QIAquick Gel Extraction Kit and QIAquick PCR & Gel Cleanup Kit for cleanup of high-quality DNA. QIAcube instruments are preinstalled with protocols for purification of plasmid DNA, genomic DNA, RNA, viral nucleic acids and proteins, plus DNA and RNA cleanup. The

WebDec 28, 2015 · PCR products were extracted using a FastGene gel/PCR Extraction Kit (Nippon Genetics, Tokyo, Japan). The extracted PCR products were ligated into pGEM-T Easy Vector (Promega), and the plasmid was introduced into Escherichia coli strain DH5α (Takara Bio Inc.,), which was plated on Luria-Bertani (LB) agar (Invitrogen, Carlsbad, … Webgel/pcr エクストラクションキットのページです。製品概要、アプリケーションノート、オーダー情報、取扱説明書、技術資料、文献、msds等、 …

WebMar 23, 2024 · The PCR products from the cDNA of the GATA2 gene were purified by cutting the band of the expected size from 1.5% agarose gels and eluting it with the FastGene Gel/PCR Extraction Kit (Nippon Genetics, Tokyo, Japan). WebFG-91302- FastGene Gel/PCR Extraction Kit (300 preps) $ 460.00. Add to cart.

WebFastGene® Gel/PCR Extraction Kits are designed for the extraction of DNA from agarose gels and for the purification of PCR products. DNA fragments purified with FastGene® Gel/PCR Extraction Kits are ready for direct use in all common downstream applications including sequencing, ligation and transformation, restriction digestion, microarray …

Webagarose gel electrophoresis, followed by extraction using FastGene Gel/PCR Extraction kit (NIPPON Genetics, Japan). A quantity of 12 fmol purified EcoRV-digested pNGS2 was combined with 60 fmol of single-stranded oligonucleotides in a volume of 5 µ L. The oligonucleotides containing randomized N 12 (12-nucleotide sequences, 5'- fun and schoolWebPCR Purification with FastGene™ Gel/PCR Extraction Kit Sample preparation. 1.Mix GP1 and PCR products in the ratio of 5 to 1 in a centrifuge tube(e.g. 200 µL of GP1 per 40 µL of PCR products). Caution: If the volume of the sample is under 40 µL, add GP1 or water for PCR products to adjust the volume of the sample to 40 µL. Sample loading girft orthopaedic pathwaysWebValidated for use with CGH labeling kit. PCR & Gel clean-up columns use silica membrane technology in a mini spin column format to deliver easy and efficient DNA purification from PCR products, agarose gels, and labeling reactions. Sample material: <400 µl PCR reaction mixture, <400mg agarose gel. Fragment size: 50bp - ~20kbp. Recovery: 70 - 95%. girft - outstandingWebSep 23, 2014 · I use PCR to amplify a 2.5-kbp gene and subsequently run the PCR products in 0.8% agarose gel. I get only a distinct band corresponding to my gene of interest. girft paediatric general surgeryhttp://www.favorgen.com/favorgen/serv_1/mem_t1/h_1/pdf/fragment/FAGCK%20000%20001%20001-1.pdf girft orthopaedics recommendationsWebApr 30, 2024 · The FastGene ® Gel/PCR Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based … girft orthopaedics 2020 girft orthopaedic trauma